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. 2006 Aug 10;576(Pt 3):809–822. doi: 10.1113/jphysiol.2006.112250

Table 1.

5-HT receptor and β-actin primers for RT-PCR

Name Sense (upper row)/ antisense (lower row) Product size (bp) Accession no.
5-HT1A 5′-TCACCTGCGACCTGTTTATC-3′ 394 J05276
5′-GCTCCCTTCTTTTCCACCTT-3′
5-HT1B 5′-TCGTGCTGGTGTGGGTCTTCT-3′ 262 X62944
5′-ATCAACTGGGCTCGGGTCAAG-3′
5-HT1D 5′-CTGAATGCTACAGGGGCTTGG-3′ 452 M89953
5′-CGGTGGGATGGAGATACAGA-3′
5-HT1F 5′-ACCTTTGGCTGAGTGTTGAC-3′ 591 L05596
5′-TAGTGGCTGCTTTGCGTTCT-3′
5-HT2A 5′-GCATCGAACTGGACAATTGATGCTGAAAA-3′ 611 M30705
5′-ATGAAAAATGCCACAAAAGAGCCTATGAG-3′
5-HT2B 5′-GTGATGCCGATTGCTCTCTT-3′ 297 X66842
5′-GATGTTGTGTGCGTTGACCA-3′
5-HT2C 5′-TTCGTTCTCATCGGGTCCTT-3′ 441 U35315
5′-CACATAGCCAATCCACACAA-3′
5-HT3 5′-GAACTACAAGCCCCTACAGC-3′ 439 D49395
5′-TGACACGATGATGAGAAAGA-3′
5-HT4 5′-GGGAGATGTTTTGCCTGGTC-3′ 484 U20907
5′-CGATGTGTGCTGTGCTGGTC-3′
5-HT5A 5′-TTCCACCGAGTACCACACAA-3′ 757 L10072
5′-TGACGGACAGTGAACACCAT-3′
5-HT5B 5′-ACGTGTGGATCTCCTTCGAC-3′ 426 L10073
5′-CAGACTCCTGAGGTGCTTCC-3′
5-HT6 5′-TACTGTAATAGCACCATGAACCCTATCAT-3′ 374 L41146
5′-CTGAGTGGATGCGGCCGTATCTCAGGCTC-3′
5-HT7 5′-AGCCCTCCAACTACCTGATT-3′ 566 L22558
5′-ACACTCTTCCACCTCCTTCT-3′
β-Actin 5′-AGCCATGTACGTAGCCATCC-3′ 294 V01217
5′-GCCATCTCTTGCTCGAAGTC-3′