Table 2.
Primer sequences used for RT-PCR and real-time PCR analysis of mRNA expression in zebrafish
| Gene | Forward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|
| (Ascension no.) | (5′–3′) | (5′–3′) | |
| CS | agcgctgctatgaatggtct | ctgaggaagacagaccctcg | 199 |
| (BC045362) | |||
| CYC-1* | acttagccaaccaggagcac | gggtggaagaagtcagaagc | 163 |
| (AY996924) | |||
| β-actin* | ttctggtcggtactactggtattgtg | atcttcatcaggtagtctgtcaggt | 170 |
| (AF057040) | |||
| HOAD | ccacaggacattcagtggtg | gtcagtgccatgaacgacag | 200 |
| (ZGI TC279717) | |||
| LDH-A* | cacagttgaagatggcctca | tgtgcgtcttgagaaacagg | 201 |
| (NM131246) | |||
| Dlat† (AY188775) | tggaactacctgccttgtcc | ctccaatgggcacatctctt | 201 |
| PGC-1α | tgaggaaaatgaggccaact | agcttcttcagcagggaagg | 200 |
| (XM678107) | |||
| PPAR-α (U93473) | gttcttcaggcggacgatt | agggggaaacacttctgaaa | 121 |
| PPAR-β1 (AF342937) | gcgtaagctagtcgcaggtc | tgcaccagagagtccatgtc | 204 |
| NRF-1 (NM131680) | aggccctgaggactatcgtt | gctccagtgccaacctgtat | 202 |
| sMyHC (AY333451) | agcccagaagcaactcaaga | gttcctgctcagccagtttc | 203 |
| fMyHC (AF165817) | aaagcaaaggccaatcttga | tgttttcctcgtgtgagctg | 200 |
CS, citrate synthase; CYC-1, cytochrome c oxidase subunit 1; HOAD, β-hydroxyacyl-CoA dehydrogenase; LDH-A, lactate dehydrogenase A subunit; dLat, pyruvate dehydrogenase E2 subunit; PPAR, peroxisome proliferator-activated receptor; PGC, PPAR-γ cofactor; sMyHC, myosin heavy chain slow isoform; fMyHC, myosin heavy chain fast isoform; NRF, nuclear respiratory factor.