Figure 4. Effect of the protein kinase C (PKC) inhibitor chelerythrine and PKC activator phorbol-12, 13-dibutyrate (PDBu) on Icat1 and Icat2 in cell-attached patches.
A, shows that 3 μm chelerythrine increased the activity of 1 nm Ang II-evoked Icat1 at −50 mV. B, 3 μm chelerythrine inhibited activity of Icat2 induced by 100 nm Ang II at −70 mV. C, shows that 1 μm PDBu reduced Icat1 activity induced by 1 nm Ang II. D, bath application of 1 μm PDBu activated Icat2 in a quiescent cell-attached patch at −70 mV. E and F, following preincubation with 3 μm chelerythrine, bath application of 100 nm Ang II (E) and 1 μm OAG (F) activated Icat1 in cell-attached patches held at −50 mV.