Table 2.
Summary of mutagenesis in HzKR127
| Mutant | Kd, nM | KdMUT/KdWT |
|---|---|---|
| CDR H1 | ||
| W33A | >10,000 | >900 |
| M34A | >10,000 | >900 |
| N35A | >10,000 | >900 |
| CDR H2 | ||
| R50A | >10,000 | >900 |
| Y52A | 276.80 ± 23.60 | 25.16 |
| P52aA | 170.30 ± 5.32 | 15.48 |
| CDR H3 | ||
| E95A | >10,000 | >900 |
| Y96A | >10,000 | >900 |
| D97A | 0.57 ± 0.03 | 0.05 |
| D97A/Y102A | 0.44 ± 0.10 | 0.04 |
| CDR L1 | ||
| L27bA | >10,000 | >900 |
| Y27dA | 1032.70 ± 56.10 | 93.90 |
| S27eA | >10,000 | >900 |
| N28A | >10,000 | >900 |
| K30A | >10,000 | >900 |
| Y32A | >10,000 | >900 |
| N34A | >10,000 | >900 |
| CDR L2 | ||
| L50A | >10,000 | >900 |
| G91A | >10,000 | >900 |
| F94A | >10,000 | >900 |
| P95A | >10,000 | >900 |
| Q96A | 293.60 ± 7.13 | 26.70 |
| WT | 11.00 ± 1.66 | |
The dissociation equilibrium constants of HzKR127 mutants (KdMUT) were measured by competitive indirect ELISA and compared with that of HzKR127 WT (KdWT).