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Total Ig level in sera of TOP3β+/+ and top3β−/− mice collected 0, 7, or 14 days after immunization. Individual mice were immunized with TNP-conjugated KLH on day 0. Each bar represents the average value from three individual TOP3β+/+ (filled bars) or three top3β−/− (empty bars) mice; error bars represent standard deviations of the samples.
