Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Rate of NADPH oxidation by CaM-bound wild-type and mutant proteins
| Protein | NADPH oxidation rate, min−1 |
||
|---|---|---|---|
| +CaM,+arginine | +CaM,−arginine | +CaM,+agmatine | |
| nNOS | 112 ± 7 | 352 ± 11 | 367 ± 28 |
| I958P nNOS | 104 ± 6 | 225 ± 12 | 246 ± 17 |
| eNOS | 39 ± 2 | 33 ± 2 | 41 ± 3 |
| P728I eNOS | 60 ± 2 | 44 ± 4 | 53 ± 5 |
| eNOS-nH | 96 ± 9 | 82 ± 3 | 94 ± 8 |
The rate of NADPH oxidation was measured at 25°C as described in Materials and Methods. The values represent the mean ± SD for three independent measurements.
