Fig. 4.

Changes in gene expression and histone modification induced by depletion of MBT. (A) Northern blot analysis of MBT-repressed E2F target genes. S2* cells were treated with RNAi of indicated E2F regulators. Total RNA was extracted from cells incubated with dsRNAs for 6 days, and the blots were probed for expression of several E2F target genes. The ethidium bromide staining of rRNA showed comparable loadings for each sample. Depletion of MBTs resulted in a strong induction in RNA expression of the selected E2F targets, pointing out their essential role in repression of endogenous E2F-regulated genes. (B) Epigenetic changes at E2F target promoters after RNAi of MBT. S2* cells were treated with RNAi of L3mbt and CG16975 for 6 days and then subjected to ChIP with antibodies recognizing dimethylated H3K9 or acetylated H4. Several E2F targets were chosen for promoter-binding analysis.