Fig. 5.
The 4-mer-induced dominant tolerance depends on endogenous lymphocytes. RAG2−/− RIP-HA mice received a first injection of 5 × 106 6.5-TCR TH1 cells, called here TH1(A), and were injected i.v. with 2.5 μg of 4-mer (filled circles) or PBS only (filled triangles). A group of mice were tolerized by the combined treatment with 4-mer and 6.5-TCR TH1 cells (filled squares), and a group of control untreated mice (filled diamonds) received on day 15 a second transfer of diabetogenic T cells, called here TH1(B). Adoptive T cell transfers are indicated by solid arrows and i.v. injections of 4-mer by dashed arrows. All PBS-treated mice (6 of 6) eventually developed fatal diabetes within 12 days of TH1 cell transfer.