Fig. 5.

MPD can be induced in transplant animals by ex vivo heat shock and cell transplantation strategy. (A) Procedure diagram. No HS donor, non-heat-shocked double transgenic donors. (B) FACS analysis of the donor cells showing that before ex vivo heat shock, the donor fish had normal blood cell numbers within the kidney marrow. (C) FACS analysis showing that primary transplant animals developed MPD 2 months after transplantation. (D and E) May–Grunwald–Giemsa-stained cytospins of the donor cells (D) and primary transplant (E). (Scale bars: 12.5 μm.)