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The unliganded VDR participates in a complex with Lef1 and β-catenin. Proteins present in extracts of COS-7 cells transfected with expression vectors for the VDR, β-catenin, and HA-tagged Lef1 were immunoprecipitated (IP) with α-HA or nonspecific mouse IgG and subjected to Western analyses. Immunoblotting (IB) was performed for β-catenin, Lef1 (Lef1-HA), and the VDR.
