Table 1.

Sequential appearance of 35 point mutations in the blood isolates

Date of isolation, month/day/year	Isolate	MIC,* μg/ml				Numeric identifier of mutation†
		Vancomycin	Rifampin	Oxacillin	Daptomycin	1‡	2§	3–6¶	7‖	8**	9††	10‡‡	11§§	12	13	14	15	16	17	18	19–33	34 and 35
7/20/2000	JH1	1.0	0.012	0.75	0.01	●																ND
9/20/2000	JH2	4.0	16	25	0.05	○	●	●	●	●												ND
10/1/2000	JH5	6.0	16	0.75	0.05	○	●	●	●	●	●											ND
10/6/2000	JH6	8.0	16	1.5	1.0	○	●	●	●	●	○	●	●	●	●	●	●	●	●	●		ND
10/13/2000	JH9	8.0	16	0.75	1.0	○	●	●	●	●	●	●	●	●	●	●	●	●	●	●	●	ND

*, E-test.

†, Numbers 1–35 are assigned to mutations in the time order of their appearance. The presence of a mutation is indicated by ●, and the absence of a mutation due to a possible reversion is indicated by ○. ND, not determined. Mutations 34 and 35 between JH1 and JH9 were predicted to be in JH9 but could not be PCR sequenced (see SI Appendix for a discussion).

‡, in blaR1 on the plasmid;

§, in SA1702 in the vraR operon;

¶, in rpoB;

‖, in rpoC;

**, in gene SA1129 with unknown function;

††, in SA1249;

‡‡, in agrC in the agr locus;

§§, in yycH in the yyc gene cluster with yycF and yycG. SAXXXX: N315 identifiers (17).