Fig. 3.

GDH overexpression with gdhA promoter-up mutations. (A) Isogenic strains SK2633 (wild type, lane 1), SK3111 (gdh-515 zch-1436::Tn10, chemostat suppressor mutation, lane 2), and SK3128 (gdh-51 zch-1436::Tn10, null mutation, lane 3) were grown in nutrient broth. Proteins of lysates from equal amounts of cells were separated by 10% SDS/PAGE and examined by Western blot. (B) Sequences of the −10 and −35 motifs of gdhA promoter in wild type, gdh-515 (from chemostat early suppressor SK3074), and gdh-611 (from plate suppressor FG1143) alleles.