Table 2.
GDH activities
| Strain | Genotype | Specific activity, nmol NADPH oxidized/min·mg of protein |
|---|---|---|
| S. typhimurium | ||
| SK2633 | WT | 124 |
| SK3128 | GDH− | 0 |
| SK3062 | GOGAT− | 306 |
| SK3074 | Chemostat suppressor | 2,350 |
| FG1143 | Plate suppressor | 2,516 |
| FG1158 | Plate suppressor | 410 |
| E. coli | ||
| NCM3722 | WT | 242 |
| FG1113 | GDH− | 2 |
| FG1079 | GOGAT− | 562 |
| FG1126 | Plate suppressor | 1,132 |
| FG1127 | Plate suppressor | 1,140 |
Strains were grown in N−C− medium with 2 mM NH4+ as N-source and 0.2% glycerol as C-source. Cultures were inoculated with overnight cultures grown in the same medium except with 5 mM NH4+. Samples were collected at similar OD650 (between 0.38 and 0.39 for S. typhimurium and between 0.27 and 0.28 for E. coli) after at least two doublings of growth.