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. 2007 May 11;5:22. doi: 10.1186/1741-7007-5-22

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Copyright © 2007 Orrego et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Displacement of histone H1 from chromatin by SAR DNA. (A) A chromatin sample containing 20 μg of DNA was titrated with a SAR fragment (657 bp). Displaced H1 formed an insoluble complex with the SAR that was separated by centrifugation. The inset shows the H1 remaining on the chromatin. The experimental points were fitted to a hyperbolic function. (B) A plot of [H1Chr]/[Chr]_freeon the ordinate and [H1SAR]/[SAR]_freeon the abscissa gives a straight line. The slope of the line corresponds to k_Chr/k_SAR. The points are the average of three experiments. The bars show the interval spanned by the extreme values.