Table 1.

Primers used in PCR/sequencing reaction of WFS1 coding region*

Primer sequence
Fragment	Forward primer (5-3)	Reverse primer (5-3)
Exon 2†	AAGCGGTGCTGGCCCATG	CCGTTCCCACCCAGCTATC
Exon 3†	TACTCCTGGCCTGGATTTGA	CATGGGCACCCTACCAACA
Exon 4†	AGTGGCCGGAGGCTCAGT	CCAACAGCATCACCAGCGT
Exon 5†	AGTCAGATGTCCATGCATCC	CTCTACAGGAAGGTTCTGGT
Exon 6†	GAGCACGCTACGTGGTGCT	GGAGGCACGGGTGAGATAG
Exon 7†	CCTGAACCCACTCAGCTC	CCAGCGGCACGGCTGTAA
Exon 8-1§	TTCCCACGTACCATCTTTCC	CACATCCAGGTTGGGCTC
Exon 8-2§	AGAACTTCCGCACCCTCAC	TCAGGTAGGGCCAATTCAAG
Exon 8-3§	CTATCGCTGCTGCCCTCC	GGGCAAAGAGGAAGAGGAAG
Exon 8-4§	GTGAGCTCTCCGTGGTCATC	CCCTCTGAGCGGTACACATAG
Exon 8-5§	ATCCTGGTGTGGCTCACG	GTAGAGGCAGCGCATCCAG
Exon 8-6§	GCGTGACTGACATCGACAAC	GCTGAACTCGATGAGGCTG
Exon 8-7§	CAGCAGCGAGTTCAAGAGC	CCTCATGGCAACATGCAC

*WFS1 sequence based on the GenBank entry (accession number: AC004599).

^†Primers were designed and synthesized herein (Methods).

^§Primers were provided by Dr. Yann-Jinn Lee.