Table 1.

Physiochemical properties and optimal reaction conditions of the proteinase K (1IC6) and the three cuticle-degrading enzymes (PR1, VCP1 and Ver112).

Protease	Fungus	Host	MWa (kDa)	NAAb	pIc	RK/DEd	EXTCOEFe (M-1cm-1)	Inhibitor	SIh (%)	Optimumi
										pH	T j (°C)
1IC6	Tritirachium album	NA	28.9	279	7.71	20/19	33200	PMSFf DFPg	100	7.5–12	50–60
PR1	Metarhizium anisopliae	Insect	28.6	281	8.37	21/18	26800	PMSF	66.4	8–10	50–60
VCP1	Pochonia chlamydosporia	Nematode	28.5	281	7.74	20/19	28650	PMSF	62.5	8–10	50–60
Ver112	Lecanicillium psalliotae	Insect	28.6	280	8.67	22/18	27370	PMSF	64.1	8–10	50–60

^a Calculated molecular weight.

^b Number of amino acids.

^c Predicted isoelectric point.

^d Number of positively charged residues/number of negatively charged residues.

^e Predicted extinction coefficient at 280 nm.

^f Phenyl methane sulphonyl fluoride.

^g Diisopropyl fluorophosphates.

^h Sequence identity with respect to 1IC6.

ⁱ Optimal reaction conditions.

^j Temperature.