Fig. 5.

Analysis of gene expression in primary mixed glia. (A) Primary mixed glial cells were isolated from WT and Lxrαβ^−/− mice, and the basal expression level of LXR target genes was analyzed. (B) Primary mixed glial cells were treated as indicated with DMSO or 1 μM GW3695 and in the presence or absence of 10 μM fAβ for 24 h. Transcript levels of the indicated genes were analyzed by quantitative PCR and are normalized to the DMSO-treated sample in each genotype. Values represent the mean ± SD of independent preparations made from WT (n = 4) and Lxrαβ^−/− (n = 4) pups. ∗, P < 0.05.