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. Author manuscript; available in PMC: 2007 Jun 14.
Published in final edited form as: Neuron. 2005 Aug;47(3):353–364. doi: 10.1016/j.neuron.2005.06.032

Figure 3. Cell-cycle kinetics of area 17 and 18 precursors.

Figure 3

A Microphotograph of E78 area 17 dissociated precursors. MAP2 (red) and PCNA (brown) immunolabeling at 4 DIV. Scale bar: 20 microns. B,C,D in vitro values B cell density (CD) values at E78. C. LI values at E62 after 3DIV D LI values at E78 after 3DIV. Values ± SEM. Statistical significance: t test. E Cartoon illustrating principles of 3H-Thy cumulative labeling. This technique is based on a continuous 3H-Thymidine exposure that leads to the incorporation of 3H-Thymidine by successive cohorts of cycling cells progressing through S phase. The projection of the LI = 100% on the x-axis gives Tc- Ts. Ts is given by the projection of LI = 0 on the x-axis. F 3H-Thy cumulative labeling at E78 in vivo. Logistic regression combined with a X2 analysis shows the two slopes are significantly different. G Cartoon showing principles of PLM (Percentage of labeled mitotic figures). This procedure, based on a brief exposure of cells to 3H-Thymidine, measures the time required for cells in S phase to enter M phase and therefore returns Tg2/m. H PLM values at E78. Statistical analysis (F test) shows the slopes are identical. I Percentage of mitoses in area 17 and area 18 OSVZ at E78. Values are ± SEM.