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All-trans retinoic acid induces formation of cystic ductal structures. Two-day pancreatic buds were cultured for 2 days (A, B, E, F), 5 days (C, D, G, H), or 3 days (I–L) without (A–D, I, J) or with 1 μM retinoic acid (RA; E–H, K, L), fixed and stained for Cytokeratin 7 (green)/DAPI (blue) (A, C, E, G), Cytokeratin 7 (green) (B, D, E, H), amylase (green)/Pan-cytokeratin (red) (I–L).
