Table 1.
Cellular profile in bronchoalveolar lavage fluid
PBS | OVA | OVA/ depletion CD25 cells | OVA/ egg antigens | |
---|---|---|---|---|
Total cells (×104/ml) | 11·5 ± 1·3 | 24·2 ± 12·5 | 35·6 ± 3·2* | 17·2 ± 4·3 |
Macrophage (×104/ml) | 11·3 ± 1·6 | 12·8 ± 8·3 | 19·2 ± 3·2* | 12·6 ± 3·2 |
Lymphocyte (×104/ml) | 0·0 ± 0·0 | 6·9 ± 3·1 | 7·7 ± 2·1 | 3·6 ± 0·2* |
Neutrophil (×104/ml) | 0·0 ± 0·0 | 0·8 ± 0·2 | 2·3 ± 0·1** | 0·2 ± 0·1 |
Eosinophil (×104/ml) | 0·0 ± 0·0 | 3·7 ± 1·8 | 6·4 ± 1·6* | 0·8 ± 0·2** |
Four groups (10/group) of mice were intratracheally administered PBS, OVA, OVA after depletion of CD25 cells, or OVA with S. japonicum egg antigen treatment for 4 week. BALF was conducted 24 h after the last intratracheal instillation. Total cell counts were determined on fresh BALF fluid and differential cell counts were assessed with Diff-Quik-staining.
P < 0·05 compared to OVA mice
P < 0·01 compared to OVA mice.