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. 2007 Jun 21;117(7):1782–1793. doi: 10.1172/JCI27523

Figure 5. Heterozygous disruption of beclin 1 decreases cardiomyocyte autophagy.

Figure 5

(A) Autophagy induced by starvation (48 hours), manifested as punctate GFP-LC3 dots, is significantly diminished in beclin 1+/– hearts. All images were taken from basal septum. Scale bar: 35 μm. (B) Quantification of GFP aggregates per microscopic field (14,479 μm2) in basal septum demonstrates significantly less autophagic activity in beclin 1+/– hearts in response to starvation compared with WT. n = 3–5 microscopic fields in each of 3 mice. (C) Induction of autophagy by pressure overload (sTAB, 48 hours) is significantly diminished in beclin 1+/– hearts compared with WT. All images are taken from basal septum. Scale bar: 35 μm. (D) Quantification of GFP aggregates per microscopic field (14,479 μm2, basal septum) demonstrated significantly less autophagic activity in beclin 1+/– hearts exposed to hemodynamic stress. n = 3–5 microscopic fields in each of 3 mice. *P < 0.05 versus α-MHC–GFP–LC3 mice.