TABLE 2.
Sensitivity of the E. coli PhoU mutant (JHU-313) and the complemented strain to antibiotics and peroxide as measured by zone of inhibitiona
Antibiotic | Concentration (μg/ml) | Inhibition zone size (mm) of indicated strain
|
|||
---|---|---|---|---|---|
W3110 | JHU-313 | JHU-313 containing pPhoU | JHU-313 containing pVector | ||
Ampicillin | 100 | 35 | 40 | 40 | 45 |
25 | 28 | 35 | 36 | 40 | |
6 | 25 | 30 | 32 | 34 | |
1.5 | 21 | 25 | 25 | 27 | |
Gentamicin | 10 | 25 | 27 | 28 | 31 |
2 | 22 | 26 | 24 | 30 | |
0.5 | 20 | 22 | 22 | 29 | |
0.1 | 14 | 20 | 17 | 22 | |
Tetracycline | 50 | 23 | 32 | 32 | 34 |
25 | 23 | 32 | 32 | 34 | |
12.5 | 22 | 26 | 30 | 34 | |
Trimethoprim | 2 | 30 | 42 | 36 | 44 |
1 | 26 | 38 | 33 | 40 | |
0.5 | 24 | 31 | 32 | 36 | |
Norfloxacin | 4 | 32 | 37 | 36 | 44 |
2 | 30 | 34 | 35 | 40 | |
0.5 | 27 | 30 | 31 | 35 | |
0.1 | 24 | 28 | 27 | 29 | |
Hydrogen peroxide (30%) | 30 | 37 | 39 | 46 |
The sensitivity of bacterial strains to antibiotics or peroxide was determined by Kirby-Bauer's method.