Figure 2.

One-dimensional NMR spectra of E2c in several conditions after solubilization in 100% D₂O. Comparison made after 30 min at atmospheric pressure in the absence of urea (Top) and after 30 min at 3 kbar (1 kbar = 100 MPa) in the absence of urea (Middle) shows that at high pressure there is considerable secondary structure as indicated by the slowly exchanging amide resonance peaks between 8 and 10 ppm. The spectrum in the presence of 5 M urea (Bottom) shows that the amide resonances are completely exchanged after 10 min.