Fig. 3.

Characterization of the peptide binding. (A) Pro-38 within the peptide is important for the formation of the curve in the peptide that prevents steric interference between the peptide and the long β-hairpin region of c-JMJD2A (β3 and β4). (B) Residues within the H3K9 and H3K36 peptides that potentially determine the specificity of peptide binding by c-JMJD2A. A proline residue at position +2 or a glycine residue at position +3 is likely to be critical for the binding. Amino acid positions are given in relation to the position of the methylated lysine residue. (C) A negative control for the demethylation assay using a mimic H3K27me2 peptide in which residues +3 and +4 from the lysine residue were mutated to Gly residues. The reaction was stopped right after the components were mixed. (D) The demethylation activity of c-JMJD2A for the mimic H3K27me2 peptide. (E) The demethylation activity of c-JMJD2D for the mimic H3K27me2 peptide.