Table 1.
Distribution of enveloped virus particles in HaCaT and Vero cells infected with HSV mutants lacking gB and gH, gB, or gH
| Virus | Cells | No. of enveloped perinuclear virions | No. of enveloped nuclear virions* | No. of cytoplasmic enveloped virions† | No. of cell surface virions | Total |
|---|---|---|---|---|---|---|
| F-BAC (wild type) | HaCaT | 9 (0.7) | 10 (0.8) | 303 (24) | 923 (74) | 1,245 |
| F-BAC gB−/gH− | HaCaT | 315 (24) | 631 (49) | 157 (12) | 194 (15) | 1,297 |
| F-BAC gB− | HaCaT | 31 (4.5) | 45 (6.5) | 152 (22) | 461 (67) | 689 |
| F-BAC gB−/gE−/gI− | HaCaT | 16 (3) | 32 (6) | 196 (36) | 298 (55) | 542 |
| F-BAC gH− | HaCaT | 14 (1.0) | 36 (2.4) | 378 (26) | 1,038 (71) | 1,466 |
| F-BAC (wild type) | Vero | 6 (1.0) | 23 (4.0) | 63 (11) | 509 (85) | 601 |
| F-BAC gB−/gH− | Vero | 412 (50) | 70 (8.5) | 173 (21) | 166 (20) | 821 |
| F-BAC (wild type) | F6/gB+12 | 7 (1.4) | 2 (0.4) | 131 (27) | 345 (71) | 485 |
| F-BAC gB−/gH− | F6/gB+12 | 14 (2.2) | 0 (0) | 143 (23) | 457 (75) | 614 |
| KOS (wild type) | HaCaT | 5 (1.2) | 8 (2.0) | 120 (25) | 277 (67) | 410 |
| KO82 gB− | HaCaT | 49 (8.2) | 48 (8.0) | 186 (31) | 311 (52) | 594 |
| SC16 (wild type) | HaCaT | 10 | 21 | ND | 602 | 633 |
| SC16 gH− | HaCaT | 22 | 45 | ND | 535 | 602 |
Enveloped HSV particles were counted in 10–15 cells. Numbers in parentheses are the percentage of total particles counted. ND, not counted.
*Enveloped particles found in herniations surrounded by a membrane within the nucleoplasm.
†The majority (>90%) of HSV particles in the cytoplasm were enveloped.