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. 2007 Jun 4;104(24):10175–10180. doi: 10.1073/pnas.0704170104

Fig. 2.

Fig. 2.

TORC2-dependent, pCREB-133-independent TCL1 activation. (A) −424luc or −m424luc reporter activity in HEK293T cells 48 h after transfection, with or without cotransfection of the mCREB (Ser-133→Ala-133) expression construct. (B) −424luc reporter activity in HEK293T cells 48 h after transfection, with or without cotransfection of the psportTORC2 (M. Montminy) expression construct. (C) Immunoblot for TORC2 repression in HEK293T cells 48 h after transfection with a TORC2 siRNA expression construct. (D) −424luc reporter activity in HEK293T cells at 48 h with or without cotransfection of a TORC2 siRNA expression construct. (E) −424luc reporter activity in HEK293T cells at 48 h with or without cotransfection of TORC2, CREB, or mCREB expression constructs. (F) −424luc reporter activity in HEK293T cells 48 h after transfection, with or without the indicated concentrations of AICAR added 6 h before assay harvest. For all experiments the data were normalized to a cotransfected Renilla luciferase (pRLCMV luciferase) reporter construct. Data represent the mean ± SD of two independent experiments, each done in triplicate.