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BCR or CD40 signaling activates CREB and represses TCL1 expression. Ramos B cells were treated with 10 μg/ml anti-IgM, 1 μg/ml anti-CD40, or 0.1 mM dbcAMP for the times indicated. (A) Immunoblot for CREB or pCREB-133 at 1 h of stimulation. (B) Northern blot for TCL1 and GAPDH with densitometry quantification. (C) Immunoblot for TCL1 and β-actin. (D) Immunoblot for CREB or pCREB-133 at 1 h of stimulation. (E) Real-time quantitative RT-PCR of TCL1 expression at 6 h of stimulation.
