Fig. 1.
BMP1 binds and cleaves PRL and GH. (A) Type I procollagen, metabolically radiolabeled with [3H]proline, was incubated in the presence (+) or absence (−) of wild-type BMP1 and/or E214A BMP1 and then subjected to SDS/PAGE and autofluorography. Flag-tagged E214A BMP1 was incubated with PRL (B) or GH (C), and proteins were immunoprecipitated with antibody against the relevant hormone followed by Western blotting with anti-Flag antibody (αFLAG) or antibody to the relevant hormone (αPRL or αGH).