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. 2007 Jun 4;104(24):9970–9975. doi: 10.1073/pnas.0701515104

Fig. 2.

Fig. 2.

Northern blot analyses of adult AenMn and AenMn2 transgenic mosquitoes. (A) Northern blot analysis conducted by using 10 μg of total RNA isolated from fully developed ovaries from untransformed Higgs strain mosquitoes (H), AenMn transgenic line 3 or AenMn2 transgenic lines 1 and 18. Duplicate membranes were hybridized with a probe specific for either the MosI ORF (MosI) or the A. aegypti nanos ORF (nos). The RNA size marker lane is indicated (M). Ethidium bromide-stained rRNA loading control of samples is shown below. (B) Northern blot analysis conducted by using 10 μg of total RNA isolated from fully developed ovaries (O), dissected carcasses (C), or whole males (♂) from AenMn and AenMn2 transgenic lines or from untransformed Higgs strain mosquitoes (H). Membranes were hybridized with a radiolabeled probe specific for the MosI ORF (MosI). rRNA loading control of samples is shown below. RNA size markers (M) are shown on the left.