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. 2007 Jun 4;104(24):9970–9975. doi: 10.1073/pnas.0701515104

Fig. 3.

Fig. 3.

RNA analysis of AenMn and AenMn2 transgenic embryos. (A) Northern blot analysis was conducted by using 10 μg of total RNA isolated from 0- to 5-h-old embryos collected from untransformed Higgs strain mosquitoes (H), AenMn lines 2, 3, 4, and 5, and AenMn2 lines 1, 9, 18, and P1. Membranes were hybridized with a radiolabeled probe specific for the MosI ORF (MosI), with rRNA loading control of samples shown below. The RNA size marker lane is indicated (M). (B) One-Step RT-PCR of 0- to 5-h-old embryos RNA collected from untransformed (H) or transgenic mosquitoes (1, 3, 4, 9, 18, and P1), using the MosI-specific forward primer MLF2 and the A. aegypti nos-specific reverse primer nosUTRR2. The arrow indicates the expected transcript size. (C) In situ hybridization of MosI in AenMn transgenic embryos. Control (H) and transgenic line (4) embryos hybridized with sense (S) or antisense (AS) nos and MosI probes. The arrow points to specific localization of the mRNA to the posterior pole.