Fig. 2.
TgRCC1 is reduced in the 73F9 mutant and nuclear-localized. (A) Total RNA from wild-type Prugniaud (WT) and 73F9 parasites was probed with a 1.6-kb cDNA fragment corresponding to the 5′ region of TgRCC1. The blot was stripped and reprobed for α-tubulin (Tub) as a loading control. (B) Total protein lysates from 5 × 106 parasites per well from Prugniaud (WT), F9 parental (F9), 73F9 mutant, and 73F9 complemented (C1H) strains. The Western blot was probed with a rat polyclonal antibody against TgRCC1. The blot was stripped and reprobed with a rabbit anti-β-tubulin antibody (Tub) as a loading control. (C) HFF cells were infected with the functional complement strain C1H that contains a C-terminal HA-tag for 24 h. Cells were incubated with anti-HA antibody to visualize the location of TgRCC1 (green) and mounted in DAPI to stain the nucleus (red).