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. 2000 Dec 19;97(26):14301–14306. doi: 10.1073/pnas.97.26.14301

Figure 4.

Figure 4

Restoration of meiosis II by Mos in starfish oocytes in which translation of mos is prevented. (A) Formation of two polar bodies along with a female pronucleus after 1-MeAde addition to oocytes that had received the injection of antisense mos (AS) and either GST-Mos or ΔN-STE11. Polar bodies (arrowheads) were detected by DAPI staining. Black arrow indicates the female pronucleus. BrdUrd incorporation was undetectable in female pronuclei of GST-Mos or ΔN-STE11-restored oocytes but could be detected in control oocytes (white arrows). (B) Two limited rounds of H1 kinase activity after 1-MeAde addition to oocytes that had received the injection of antisense mos and the GST-Mos fusion protein. After 1-MeAde addition, oocytes were recovered at indicated times and processed for measurement of H1 kinase activity (Top and Middle for autoradiogram; Bottom for radioactivity of the excised histone H1 bands; open circles, injection with antisense mos plus GST-Mos; closed squares, injection with antisense mos plus GST) and for Tyr phosphorylation in Cdc2 (Bottom, Insets). In both A and B, control oocytes had been injected with antisense mos (AS) and GST before 1-MeAde addition.