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. 2007 Feb 1;25(4):1097–1111. doi: 10.1111/j.1460-9568.2007.05344.x

Fig. 3.

Fig. 3

Characterization of sciatic nerve transection (SCNT)-induced cell adhesion molecule L1 (L1-CAM)-immunoreactive (ir) ring structure in dorsal root ganglia (DRGs). (A–D) Double staining of L1-CAM-ir profiles (red) with glial fibrillary acidic protein (GFAP) (green), a marker of satellite cells and Schwann cells, in the L5 DRGs 7 days after SCNT. (C and D) Merged images of L1-CAM-ir profiles and GFAP-ir staining. (D) Higher magnification image of C. Arrows indicate the L1-CAM-ir profiles that are located in neuronal somata. (E) Double staining of L1-CAM (black) with activating transcription factor 3 (ATF3) (brown), a marker of injured neurons, shows the L1-CAM-ir ring structures formed around injured DRG neurons. (F–I) Triple staining demonstrates heavy colocalization of L1-CAM (red) with phospho-p38 MAPK (p-p38) (green) and not with NF-200 (purple), a marker for myelinated A fibers in injured DRG neurons. Scale bars: A–C, 10 µm; D, 2.5 µm; E, 25 µm; F and G, 25 µm.