Table 3.

PCR confirmations of OCT4 and SRY target genes

Two different antibodies were used to prepare SRY ChIP samples for confirmation PCR assays; the targets in this list were identified originally by ChIP-chip using the SRY polyclonal antibody.

^aThe values in the table represent the fold enrichment, which was compared to the enrichment of the total input and normalized to the negative control. The signals were within the linear range of the assay, providing a semiquantitative analysis. For each of these experiments, IgG ChIP samples were performed; the promoters did not show enrichment in the IgG samples.