TABLE 1.
E. faecalis strains used in time-kill and synergism studies
Strain | Relevant characteristic(s) | Reference | Bla+ | MIC (μg/ml)
|
HLR to aminoglycosides
|
Synergisma | |||
---|---|---|---|---|---|---|---|---|---|
BPR | AMPd | VANe | GENf | STRg | |||||
TX0630 | Blood isolate from Argentina recovered in 1990 | 4 | Yes | 0.25 | 1 | 0.5 | Yes | No | Yes |
TX5070 | JH2-7 transconjugant resulting from mating with a bla+ strain (HH-22), thymine auxotroph | 12 | Yes | 0.125b | 8b | 8b | Yes | No | Yes |
TX2484 | Houston blood isolate, 1994, VanB | 4 | No | 0.5 | 0.5 | >256 | No | Yes | Yes |
TX2784 | Human fecal isolate from Spain, 1998, VanA | 18 | No | 0.25 | 0.5 | >256 | No | Yes | Noc |
With either 10 μg/ml gentamicin or 25 μg/ml streptomycin when appropriate.
Determined with BHI agar since TX5070 does not grow on Mueller-Hinton agar. E. faecalis ATCC 29212 was used as a control.
Decrease in the number of CFU per milliliter of >1 log10 but <2 log10 versus BPR at 24 h.
AMP, ampicillin.
VAN, vancomycin.
GEN, gentamicin.
STR, streptomycin.