TABLE 2.

Residual activities estimated as initial rates of substrate hydrolysis of MBLs after 30 min of preincubation at room temperature with 2-picolinic acid and its derivatives^a

Enzyme	Residual activity (%) in the presence of:
	No inhibitor	2,3-PDCA	2,4-PDCA	2,5-PDCA	2,6-PDCA	3,4-PDCA	2-Picolinic acid
IMP-1 with no added Zn	100	89 ± 2	94 ± 4	99 ± 4	2 ± 4	91 ± 5	31 ± 8
IMP-1	100	67 ± 3	78 ± 3	81 ± 2	72 ± 2	88 ± 7	54 ± 6
BcII	100	98 ± 4	97 ± 3	99 ± 2	95 ± 3	97 ± 3	95 ± 5
VIM-2	100	106 ± 6	98 ± 5	97 ± 3	96 ± 8	92 ± 3	80 ± 2
VIM-4	100	73 ± 3	90 ± 1	97 ± 2	88 ± 2	100 ± 1	59 ± 2
CphA with no added Zn	100	130 ± 10	38 ± 4	114 ± 14	12 + 17	116 ± 3	8 ± 18
L1	100	93 ± 2	78 ± 2	94 ± 5	92 ± 14	97 ± 2	29 ± 13
FEZ-1	100	62 ± 4	65 ± 6	80 ± 11	77 ± 6	93 ± 8	62 ± 2

^aThe reporter substrates used were nitrocefin (FEZ-1) and imipenem (all other enzymes) at a concentration of 100 μM. Residual activity was tested in the presence of 100 μM inhibitor. Values are means ± standard deviations.