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pH dependence of the kinetic constants Km and kcat for wild-type CphA by using the substrate imipenema
| pH | Km (μM) | kcat (s−1) | kcat/Km (μM−1 s−1) |
|---|---|---|---|
| 7.0 | 120 ± 10 | 210 ± 8 | 1.8 |
| 8.0 | 83 ± 6 | 140 ± 4 | 1.7 |
| 9.0 | 63 ± 3 | 49 ± 1 | 0.78 |
| 10.0 | ND | ND | 0.44 |
Values are means ± standard deviations. ND, not determined (see text).
