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Alignment of 21 genotype 1b sequences in the primer regions. The M414-S and M414-AS primers used for the first-round PCR are indicated by arrows at the top of the sequences. The arrow at the bottom of the right-hand sequence indicates the location of the antisense primers used in the allele-specific real-time PCRs. The shaded nucleotide indicates the position that differs between the 414M and 414T primers.
