FIG. 2.
(A) Equal amounts of mutant or wild-type standard DNA templates were amplified with mutant-specific primers (upper panel) or wild-type-specific primers (lower panel), respectively. Both assays have a discriminatory ability of about 11 cycles. (B) Discriminatory ability of M414T-specific real-time PCR. A total of 104 (upper panel) or 105 (lower panel) copies of wild-type standard DNA were added to each serial dilution (108 to 101 copies) of mutant standard DNA. In each case, the CT was compared to PCRs performed without the addition of wild-type DNA. •, M414T standard only; ×, M414T mixed with 104 (upper panel) or 105 (lower panel) copies of wild-type DNA.