TABLE 4.
NS5B mutations in TA clonesa
| TA clone | NS5B mutations |
|---|---|
| 1 | Y296C, A334V |
| 2 | M71I, S113R, Q148R, A252V, C366R, I392T, M414T, L459P, A553T |
| 3 | M414T, Y586C |
| 4 | R280C, E361V, G554D |
| 5 | M414T, Q514R |
| 6 | R32C, A75V, Q241R, V281V, M414T |
| 7 | K270R, Y448H, P495L |
| 8 | Y448H, P495L |
| 9 | K270R, Y448H, P495L |
| 10 | G378D, M414T, L525P |
| 11 | Y448C, Y586C |
| 12 | M414T, Y586C |
| 13 | I23T, G351E, M414T |
| 14 | L60P, V67A, M414T, S513P, N590S |
| 15 | C146R, C445R, S540T, G554D |
a
TA clones contained NS5B genes amplified by RT-PCR. RNA was extracted from replicon cells that had been treated with 100× above the EC50 (7,000 nM) of A-782759 for 4 days and then continually cultured without drug for another 24 days. Mutation M414T is indicated in boldface.