TABLE 3.
Oligonucleotide primers used for PCR mapping and sequencing of the Tn3-like elements carrying the blaTEM-92 gene
Primer no. | Primer name | Sequence (5′-3′) |
---|---|---|
1 | IR tn1-3 | TGACGCTCAGTGGAACGAA |
2 | tnpA-tn3/F | GCATGTTCGTACCTGCTGA |
3 | tnpA-tn3/R | TCAGCAGGTACGAACATGC |
4 | tnpR-tn3/F | CCAGTCAGCAGTCTCTTGA |
5 | tnpR-tn3/R | GGCAATACTGAGCTGATGAG |
6 | IS15/26 | CCACCATCAAAGGTATTGAG |
7 | pFOR-TEM | ATAAAATTCTTGAAGACGAA |
8 | Rev-TEM | ATATGAGTAAGCTTGGTCTGACAG |