TABLE 2.
Phosphorylation of nucleoside analogs (100 μM) by BV TKa
| Analog | Phosphorylation (%)b (mean ± SD) |
|---|---|
| TdR | 100 |
| l-TdR | 130 ± 8.0 |
| TFT | 55 ± 4.2 |
| BVdU | 0 |
| l-BVdU | 0 |
| IUdR | 58 ± 2.5 |
| l-IUdR | 94 ± 4.3 |
| Et-dU | 82 ± 4.1 |
| Pr-dU | 31 ± 2.5 |
| ACV | 0 |
| GCV | 0 |
| CFV | 0 |
| PCV | 72 ± 5.1 |
| HBPG | 200 ± 25 |
| HBG | 94 ± 7.2 |
a
Determined by HPLC analysis of reaction mixtures containing BV TK after 30 min of incubation.
b
Relative to the mean of TdR, 0.5 nmol TMP produced in the assay reaction conditions (see Materials and Methods). Zero values indicate no detectable monophosphate at 100 μM or 1 mM.