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Kinetic properties of phosphorylation of substrates by BV TKa
| Substrate | Km (μM) (mean ± SD)b | Vmax (nmol h−1 μg−1) (mean ± SD)b | Vmax/Km |
|---|---|---|---|
| TdR | 0.70 ± 0.05 | 0.20 ± 0.005 | 0.28 |
| HBPG | 7.40 ± 0.047 | 3.90 ± 0.1 | 0.52 |
| l-TdR | 0.65 ± 0.15 | 0.15 ± 0.008 | 0.23 |
Assayed at variable concentrations of substrate.
The values are the means from two independent experiments in which each concentration was tested in duplicate.
