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. 2007 Mar 19;51(6):2215–2218. doi: 10.1128/AAC.01368-06

FIG. 2.

FIG. 2.

Testing of siRNA using the Lassa virus replicon system. BSR-T7/5 cells were transfected with components of the Lassa virus minireplicon system (Ren-Luc), a control plasmid expressing firefly luciferase, and 10 pmol of Lassa virus-specific or nonsense (nons.) control siRNA. Minigenomes with three different termini upstream of the reporter gene were used for testing: with NP gene-specific terminus for testing NP, NP-A, NP-B, and nonsense 1 siRNA; with GPC/Z gene-specific terminus for testing GPC/Z and nonsense 2 siRNA; and with L gene-specific terminus for testing L and nonsense 3 siRNA. Renilla and firefly luciferase activity were measured 24 h posttransfection. Replicon activity is expressed as the Renilla/firefly luciferase ratio. Replicon activity in the presence of nonsense siRNA was set at 1. Mean and range of duplicate transfections are shown.