. 2007 Mar 19;51(6):1946–1955. doi: 10.1128/AAC.01412-06

TABLE 2.

Primers used for PCR amplification and sequencing

Gene	Primer^a	Primer sequence (5′→3′)	PCR product size (bp)	Source or reference
bla_TEM	P1	TACGATACGGGAGGGCTTAC	716	2
	P2	TTCCTGTTTTTGCTCACCCA
	FIN	ATTCTTGAAGACGAAAGGGC	1,091	7
	DEB	ATGAGTAAACTTGGTCTGAC
	P3^b	TGGGTGAGCAAAAACAGGAA		7
	CLB^b	AATGAAGCCATACCAAACGA		2
bla_SHV	SHVf1	TCAGCGAAAAACACCTTG	471	32
	SHVr2	TCCCGCAGATAAATCACCA
bla_OXA	oxa1f	TATCTACAGCAGCGCCAGTG	199	17
	oxa1r	CGCATCAAATGCCATAAGTG
	oxa1sf	ATGAAAAACACAATACATATC	816	9
	oxa1sr	AATTTAGTGTGTTTAGAATGG
ampC	ampCf	CCCCGCTTATAGAGCAACAA	634	17
	ampCr	TCAATGGTCGACTTCACACC
bla_CTX-M	CTXf^c	TTTGCGATGTGCAGTACCAGTAA	543	15
	CTXr^d	CGATATCGTTGGTGGTGCCATA
bla_CTX-M-15	CTX15f	AGAATAAGGAATCCCATGGTT	875	This study
	CTX15r	ACCGTCGGTGACGATTTTAG
	CTX15i^b	GGAATCTGACGCTGGGTAAA		This study
bla_CTX-M-14	CTXg3f	CTGATGTAACACGGATTGACC	871	This study
	CTX14r	CGATTTATTCAACAAAACCAG
ISEcp1	ISEcp1	AAAAATGATTGAAAGGTGGT	Variable	14
IS26	IS26	AGCGGTAAATCGTGGAGTGA	Variable	14
IS903	IS903	CGGTTGTAATCTGTTGTCCA	Variable	14

For PCR amplification and sequencing (except those in used only for internal sequencing).

Used only for internal sequencing.

When used with primers ISEcp1, IS26, IS903, this also amplifies the downstream region of the bla_CTX-M gene. Various-sized PCR products are expected.

When used with primer ISEcp1, IS26, or IS903, this also amplifies the upstream region of the bla_CTX-M gene. Various-sized PCR products are expected.