FIG. 1.

Schematic gene organization of genome-length HCV RNA. (A) ORN/C-5B/KE RNA replicated in OR6 cells. RL (RLuc) is expressed as a fusion protein with neomycin phosphotransferase (NeoR). The position of an adaptive mutation, K1609E, is indicated by a black triangle. (B) Authentic HCV RNA, HCV-O/KE/EG, was introduced into the OR6c cells by electroporation as previously described (13). The positions of two adaptive mutations, E1202G and K1609E, are indicated by black triangles. IRES, internal ribosome entry site; EMCV, encephalomyocarditis virus.