TABLE 2.
Kinetic constants of squalene synthase from different species and sources
| Species and/or sourcea | SQS type | FPP
|
NADPH
|
kcat (s−1) | Reference | ||
|---|---|---|---|---|---|---|---|
| Km (μM) | Vmax (nmol min−1 mg−1) | Km (μM) | Vmax (nmol min−1 mg−1) | ||||
| Human pHSS12/pHSS16 | Double-truncated SQS | 2.8 | 1.44 | 21 | |||
| T. cruzi | Glycosomal SQS | 2.8 | 0.97 | 40 | 0.43 | 25 | |
| T. cruzi | Microsomal/mitochondrial SQS | 2.3 | 1.18 | 33 | 0.76 | 25 | |
| L. mexicana | Glycosomal SQS | 3.2 | 0.75 | 62 | 0.58 | 25 | |
| L. mexicana | Microsomal/mitochondrial SQS | 2.8 | 0.98 | 57 | 0.84 | 25 | |
| Human | Full-length SQS expressed in a baculoviral system | 2.3 ± 0.5 | 4,800 ± 460 | 430 ± 60 | 3,800 ± 150 | 19 | |
| Yeast | C-terminal truncated SQS | 2.5 ± 0.46 | 530 ± 77 | 0.53 ± 0.03 | 12 | ||
| T. cruzi | |||||||
| TcSQS24/36 | 5.25 ± 1.2 | 1,428.56 ± 317 | 23.34 ± 4.5 | 1,853.24 ± 435 | 1.05 ± 0.16 | ||