TABLE 1.
Effect of 3TC and DFC combinations against HIV-1LAI-infected human PBM cells
| Treatment (ratio) | EC50a | EC90a | CI ± SE at Fab of:
|
|||
|---|---|---|---|---|---|---|
| 0.50 | 0.75 | 0.90 | 0.95 | |||
| Expt 1 | ||||||
| 3TC | 0.06 | 0.32 | ||||
| DFC | 0.35 | 2.22 | ||||
| DFC + 3TC (100:1) | 0.26 | 1.99 | 0.80 (0.77) | 0.90 ± 0.63 (0.86 ± 0.58) | 1.00 ± 0.43 (0.95 ± 0.41) | 1.08 ± 0.39 (1.02 ± 0.32) |
| DFC + 3TC (25:1) | 0.29 | 1.32 | 1.15 ± 0.65 (1.00 ± 0.72) | 0.97 ± 0.46 (0.85 ± 0.53) | 0.82 ± 0.32 (0.73 ± 0.39) | 0.73 ± 0.25 (0.65 ± 0.31) |
| Expt 2 | ||||||
| 3TC | 0.026 | 0.12 | ||||
| DFC | 0.075 | 0.23 | ||||
| DFC + 3TC (10:1) | 0.003 | 0.092 | 0.05 ± 0.04 (0.05 ± 0.04) | 1.14 ± 0.08 (0.14 ± 0.07) | 0.46 ± 0.22 (0.43 ± 0.17) | 1.04 ± 0.61 (0.93 ± 0.40) |
| DFC + 3TC (3:1) | 0.059 | 0.28 | 1.48 ± 0.68 (1.15 ± 0.44) | 1.72 ± 0.71 (1.30 ± 0.42) | 2.02 ± 0.81 (1.50 ± 0.50) | 2.27 ± 0.95 (1.65 ± 0.57) |
a
In μM as determined from the median effect plot.
b
A CI of <1, equal to 1, or >1 indicates synergy, additivity, or antagonism, respectively. Fa is a component of the median effect equation referring to the fraction of the system (e.g., 0.50 means the CI at a 50% reduction of RT activity). CI values were determined for a mutually nonexclusive interaction (values in italics are for mutually exclusive interaction, which is less rigorous).