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(A) SDS-PAGE of recGlNR1 in noninduced (C) or induced (I) (3.5 h, 1 mM IPTG) E. coli and after His tag purification (HT). Lane M, sizes in kilodaltons. (B) Activity of recGlNR1 with DNT, NTZ, or MET as a substrate (50 μM). Assays were run in triplicate. Mean values ± SE are given.
