TABLE 2.
Comparison of IC50 values determined using the MSF and [3H]hypoxanthine incorporation assays at 72 h of incubation
| Strain and test drug | Mean IC50 (ng/ml) ± SEMa as determined by:
|
|||||
|---|---|---|---|---|---|---|
| MSF assay
|
[3H]HPX assay | |||||
| TCM | TCMA | FAF | FAFA | Combined | ||
| D6 | ||||||
| Chloroquine | 9.4 ± 0.8 (6) | 10.4 ± 1.1 (4) | 8.1 ± 0.8 (12) | 8.1 ± 0.6 (13) | 8.6 ± 0.4* (35) | 4.1 ± 0.1 (136) |
| Mefloquine | 43.7 ± 4.1† (6) | 19.9 ± 5.1† (4) | 32.8 ± 2.1 (7) | 16.0 ± 3.0† (5) | 29.6 ± 3.0* (22) | 4.5 ± 0.3 (78) |
| Quinine | 15.8 ± 1.6 (6) | 11.3 ± 1.7 (4) | 14.9 ± 0.5 (7) | 11.9 ± 1.2 (5) | 13.8 ± 0.7* (22) | 5.0 ± 1.0 (3) |
| Artemisinin | 1.25 ± 0.17 (6) | 1.7 ± 0.27 (4) | 1.7 ± 0.22 (7) | 1.6 ± 0.18 (3) | 1.5 ± 0.1* (20) | 2.4 ± 0.2 (24) |
| Doxycycline | 1,175.3 ± 38.8 (3) | 2,371.5 ± 406.1 (4) | 856.9 ± 62.6 (6) | 1,845.8 ± 484.1 (5) | 1,521.3 ± 207.8 (18) | 483.3 ± 54.5 (6) |
| Azithromycin | 10,134.3 ± 697.6 (6) | 15,277.0 ± 1193.5 (4) | 10,073.4 ± 666.4 (10) | 14,364.7 ± 640.5 (9) | 12,135.5 ± 556.2* (29) | 3,746.8 ± 636.7 (30) |
| Pyrimethamine | ND | ND | 0.5 ± 0.2 (6) | 0.4 ± 0.1 (8) | 0.4 ± 0.1 (14) | 0.1 ± 0.1 (14) |
| Dapsone | ND | ND | 3.1 ± 0.4 (4) | 10.2 ± 1.5 (4) | 6.7 ± 1.5 (8) | 2.9 ± 0.4 (8) |
| Sulfadoxine | ND | ND | 10.5 ± 7.2 (5) | 2.4 ± 1.1 (4) | 6.9 ± 4.1 (9) | 16.0 ± 4.7 (15) |
| W2 | ||||||
| Chloroquine | 248.5 ± 29.2 (4) | 331.5 ± 30.3 (3) | 274.6 ± 20.0 (10) | 267.7 ± 26.9 (10) | 274.5 ± 13.6* (27) | 130.4 ± 3.3 (137) |
| Mefloquine | 16.2 ± 2.0 (3) | 8.9 ± 1.2 (3) | 17.4 ± 3.0 (9) | 9.7 ± 0.7 (3) | 14.5 ± 1.7* (18) | 2.3 ± 0.1 (78) |
| Quinine | 167.5 ± 17.1 (4) | 128.5 ± 25.3 (3) | 162.3 ± 20.4 (9) | 92.3 ± 3.1 (3) | 147.0 ± 12.2* (19) | 36.0 ± 9.5 (3) |
| Artemisinin | 1.0 ± 0.19 (4) | 1.4 ± 0.4 (4) | 1.2 ± 0.4 (7) | 1.1 ± 0.03 (3) | 1.2 ± 0.2 (18) | 1.7 ± 0.2 (27) |
| Doxycycline | 2,413.3 ± 438.5 (3) | 2,281.3 ± 310.5 (4) | 1,863.0 ± 423.4 (4) | 2,134.7 ± 343.6 (3) | 2,158.6 ± 178.2* (14) | 814.0 ± 57.8 (5) |
| Azithromycin | 1,623.3 ± 267.5 (3) | 2,733.6 ± 502.4 (5) | 1,847.9 ± 375.5 (7) | 4,488.8 ± 526.6 (8) | 2,929.7 ± 344.0 (23) | 5,500.4 ± 1,053.8 (23) |
| Pyrimethamine | ND | ND | 8.3 ± 4.8 (3) | 37.6 ± 9.8 (4) | 25.0 ± 8.1 (7) | 11.9 ± 2.1 (7) |
| Dapsone | ND | ND | 156.2 ± 23.9 (3) | 241.6 ± 27.8 (4) | 204.9 ± 24.5* (7) | 124.1 ± 7.3 (9) |
| Sulfadoxine | ND | ND | 1,642.1 ± 674.0 (3) | 2,417.2 ± 888.3 (3) | 2,107.1 ± 564.1 (5) | 1,112.9 ± 192.1 (10) |
a
Means of experiments run on different days are given. The “Combined” data column represents the means of all of the TCM, TCMA, FAF, and FAFA data together for each respective test drug. *, P < 0.05 compared to the [3H]hypoxanthine (HPX) incorporation assay; †, P < 0.05 compared to the MSF “Combined” data. ND, not determined.